Yazar "Çanakcı, Sabriye" için listeleme
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Antimicrobial activity of Bacillus strains isolated from spring water and a novel bacteriocin: RS108
The aim of this research was to investigate the bacteriocins produced by Bacillus strains isolated from the spring water of Rize, in Turkey. Bacillus cereus RS108 was identified by both conventional and molecular methods, ... -
The ATPase activity of the G2alt gene encoding an aluminium tolerance protein from Anoxybacillus gonensis G2
Beriş, Fatih Şaban; De Smet, Lina; Karaoğlu, Hakan; Çanakcı, Sabriye; Van Beeumen, Jozef; Belduz, Ali Osman (Springer, 2011)The G2ALT gene was cloned and sequenced from the thermophilic bacterium Anoxybacillus gonensis G2. The gene is 666 bp long and encodes a protein 221 amino acids in length. The gene was overexpressed in E. coli and purified ... -
Biochemical characterization of a novel glucose isomerase from Anoxybacillus gonensis G2(T) that displays a high level of activity and thermal stability
Karaoğlu, Hakan; Yanmış, Derya; Sal, Fulya Ay; Çelik, Ayhan; Çanakcı, Sabriye; Belduz, Ali Osman (Elsevier Science Bv, 2013)In the continuing search for novel enzymes suitable for the production of high fructose corn syrup (HFCS), a new glucose isomerase (GI) from the thermophile Anoxybacillus gonensis G2(T) is described. the gene encoding this ... -
Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2(T)
Yanmış, Derya; Karaoğlu, Hakan; Çolak, Dilşat Nigar; Sal, Fulya Ay; Çanakcı, Sabriye; Belduz, Ali Osman (Tubitak Scientific & Technical Research Council Turkey, 2014)The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2(T) has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography ... -
Cloning, expression, purification and characterisation of a thermostable chitinase from Bacillus licheniformis A1
Sandallı, Cemal; Kaçağan, Murat; Çanakcı, Sabriye; Belduz, Ali Osman (University of Milan, 2008)The chitinase B gene (ch/B65) of Bacillus licheniformis A1 (BlicA1) isolated from Diyadin hotspring in Turkey was cloned and sequenced. The gene is 1779 bp long and encodes a protein 592 amino acids with a 35-amino acid ... -
Cloning, expression, purification and characterization of fructose-1,6-bisphosphate aldolase from Anoxybacillus gonensis G2
Ertunga, Nagihan Sağlam; Çolak, Ahmet; Belduz, Ali Osman; Çanakcı, Sabriye; Karaoğlu, Hakan; Sandallı, Cemal (2007)The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein ... -
Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1
Ay, Fulya; Karaoğlu, Hakan; İnan, Kadriye; Çanakcı, Sabriye; Belduz, Ali Osman (Elsevier, 2011)The gene encoding a carboxylesterase from Anoxybacillus sp., PDF1, was cloned and sequenced. The recombinant protein was expressed in Escherichia coli BL21, under the control of isopropyl-?-d-thiogalactopyranoside-inducible ... -
Comparison of DIG-11-dUTP utilization by Geobacillus caldoxylosilyticus TK4, Mycobacterium tuberculosis and Escherichia coli DNA polymerases
Sandallı, Cemal; Çanakcı, Sabriye; Demir, İsmail; Modak, Mukund J.; Belduz, Ali Osman (Springer Link, 2010)DNA polymerases are used for many applications and we comparatively investigated DNA synthesis activity of DNA polymerase I enzymes of Geobacilluscaldoxylosilyticus TK4, Escherichia coli and Mycobacterium tuberculosis with ... -
Molecular analysis of the genus Anoxybacillus based on sequence similarity of the genes recN, flaA, and ftsY
Çolak, Dilşat Nigar; İnan, Kadriye; Karaoğlu, Hakan; Çanakcı, Sabriye; Beldüz, Ali Osman (Kluwer Academic Publishers, 2012)Genome predictions based on selected genes would be a very welcome approach for taxonomic studies. We analyzed three genes, recN, flaA, and ftsY, for determining if these genes are useful tools for systematic analyses in ... -
Sulfonamide inhibition studies of the beta-carbonic anhydrase from the newly discovered bacterium Enterobacter sp B13
Eminoğlu, Ayşenur; Vullo, Daniela; Aşık, Aycan; Çolak, Dilşat Nigar; Çanakcı, Sabriye; Beldüz, Ali Osman; Supuran, Claudiu T. (Pergamon-Elsevier Science Ltd, 2016)The genome of the newly identified bacterium Enterobacter sp. B13 encodes for a beta-class carbonic anhydrases (CAs, EC 4.2.1.1), EspCA. This enzyme was recently cloned, and characterized kinetically by this group (J. ... -
Use of rpoB sequences and rep-PCR for phylogenetic study of Anoxybacillus species
İnan, Kadriye; Bektaş, Yusuf; Çanakcı, Sabriye; Belduz, Ali Osman (Springer, 2011)This study was conducted to investigate the applicability of rpoB, which encodes the ? subunit of RNA polymerase, to be used as an alternative to 16S rRNA gene sequence similarity analysis in the thermophilic genus ...